GROWTH CURVE OF HELA STRAIN CELLS IN TISSUE CULTURE

An Unusual Behavior of Strain Hela Cells in Tissue Culture

The differentiation attained by emigrating embryo chick ceils cultivated under strips and sheets of dialysis cellphane (1-4) in multipurpose culture chambers (5) prompted us to make a similar study of malignant tissues and strains. One of the more provocative results obtained with HeLa cells (6, 7) is contained in this note. The environmental conditions resulting when sheets of cellophane are l...

Survival of Bordetella pertussis in Tissue Culture of Animal Cells

The Specific Amino Acid Requirements of a Human Carcinoma Cell (strain Hela) in Tissue Culture

The amino acid requirements of a human uterine carcinoma cell (HeLa strain) have been defined. The 12 compounds previously found to be essential for the growth of a mouse fibroblast proved similarly essential for this human epithelial cell. They included arginine, cyst(e)ine, histidine, and tyrosine, in addition to the eight amino acids required for nitrogen balance in man (isoleucine, leucine,...

A Comparison of the Growth of Selected Mycobacteria in Hela, Monkey Kidney, and Human Amnion Cells in Tissue Culture

HeLa, monkey kidney, and human amnion cells in tissue cultures were compared as sites for the multiplication of strains of tubercle bacilli or original and reduced pathogenicity, and for several other species of mycobacteria capable of causing disease in humans. The arrangement of the pathogenic species inorder of their growth rates in HeLa cells was Mycobacterium fortuitum, Mycobacterium balne...

Physiological and biochemical studies of effects of mitomycin C on strain HeLa cells in cell culture.

from Streptomyces cae@pitoaua(i), has been demon strated to have strong antitumor action not only on solid, ascitic, and leukemic tumors of the mouse, the rat, and the hamster (3, 10, 13, 16, 18), but also on various types of human cancer (14, 17). It was also shown by Perlman and his associates (8) that this antibiotic was highly toxic against the mouse fibroblast strain L cells cultured in vi...